This study was conducted to determine the quality of honey produced in Silti district of southern Ethiopia. The honey samples were collected from different locations and represented two agro-ecologies: Dega (highland) and Weyna Dega (midland). From each of these locations honey samples were obtained from traditional and movable frame hives. In addition, honey samples were also collected from local market. Analysis of physicochemical parameters of honey was carried out at the Health and Nutrition Research Institute and Quality and Standards Authority of Ethiopia. The honey samples analyzed had average moisture, ash, free acidity, water insoluble matter, pH, reducing sugars and sucrose contents of 15.95 (g/100g), 0,34 (g/100g), 19.32 (meq/kg), 0.26 (g/100g), 4.45, 69.04 (g/100g) and 4.1 (g/100g), respectively. Comparison of quality parameters of honey samples from the two agro-ecologies and the market showed that market honey samples had significantly (P<0.05) higher reducing sugars content than honey samples from the two agro-ecologies, but no significant difference (P>0.05) was observed between market honey and honey samples from the two agro-ecologies for the remaining parameters. All the quality parameters analyzed for honey produced in Silti district comply with both national and international standards except water-insoluble matter which was high in honey samples collected from traditional hives. Thus, there is a need to educate beekeepers of the area about proper honey harvesting, straining and handling techniques.